DAPK3 antibody RabMAb®

Cat.#: 2034-1
Abcam ID: ab51602

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).

Recommended Applications

WB, FC

Applications and Recommended Dilution Factors

WB: 1:500
FC: 1:10

Species Reactivity *

Human

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on HeLa cell lysate using anti-DAPK3 RabMAb (cat. # 2034-1).

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Specificity

A synthetic peptide corresponding to residues on the C-terminus of human DAPK3 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

DAPK3 antibody, ZIP antibody, ZIPK antibody, Death-associated protein kinase 3 antibody, MYPT1 kinase antibody, Zipper-interacting protein kinase antibody

Description References

1. Kawai T, et al. Mol Cell Biol 18(3):1642-51, 1998
2. Kawai T, et al. Mol Cell Biol 23(17):6174-86, 2003
3. Preuss U, et al. Nucleic Acids Res 31(3):878-85, 2003
4. Murata-Hori M, et al. FEBS Lett 451(1):81-4, 1999

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