PPT1 Antibody (N-term)Cat.#: T0829
Abcam ID: ab135516
Rabbit Polyclonal Antibody
Swiss Prot: P50897
Mol Weight: 34kDa
Price: Contact Abcam
Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
Applications and Recommended Dilution Factors
|Western blot analysis of hPPT1-C46 in mouse cerebellum tissue lysates (35ug/lane).PPT1 (arrow) was detected using the purified Pab.|
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~69 amino acids from the N-terminal region of human PPT1.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
PPT1 antibody, CLN1 antibody, INCL antibody, PPT antibody, Palmitoyl-protein thioesterase 1 antibody, Palmitoyl-protein hydrolase 1 antibody
Calero, G., et al., J. Biol. Chem. 278(39):37957-37964 (2003).
Hofmann, S.L., et al., Curr. Mol. Med. 2(5):423-437 (2002).
Weimer, J.M., et al., Neuromolecular Med. 1(2):111-124 (2002).
Lu, J.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 93(19):10046-10050 (1996).
Crews, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 93(9):4316-4319 (1996).
|Product Name||Catalog #|
|PPT1 Antibody (C-term)||T0830|
|Product Name||Catalog #|
|Goat Anti-Rabbit IgG (H+L) HRP||3053-1|
|Goat Anti-Rabbit IgG polymerized HRP||3051-1|
|Cell Lysis Buffer||3500-1|
|Goat Anti-Rabbit IgG HRP Antibody||3056-1|