p16-Arc (N-term) (ARPC5) antibody RabMAb®

Cat.#: 1961-1
Abcam ID: ab51243

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3, and five others referred to as p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc (p complex) (1). These proteins localize in the cytoplasm of fibroblasts that lack lamellipodia, but are enriched in lamellipodia on stimulation with serum or platelet-derived growth factor. A dynamic role for this complex in the organization of the actin cytoskeleton has been suggested (2). p16-Arc has been found to be a globular alpha-helical subunit (3).

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:50 - 100
ICC: 1:50 - 100
IP: 1:70
FC: 1:20

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on fetal brain lysate using anti-p16 Arc RabMAb (cat. # 1961-1), dilution 1:20,000	B. Immunohistochemical analysis of paraffin-embedded human uterus adenocarcinoma using anti-p16-ARC RabMAb (cat. #1961-1).

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Specificity

A synthetic peptide corresponding to residues on the N-terminus of human p16-ARC was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ARPC5 antibody, ARC16 antibody, dJ127C7.3 antibody, p16-Arc antibody, Actin-related protein 2/3 complex subunit 5 antibody, Arp2/3 complex 16 kDa subunit antibody

Description References

1. Welch MD, et al. J Cell Biol 138(2):375-84, 1997
2. Machesky LM, et al. Biochem J. 328(Pt1):105-12, 1997
3. Robinson RC, et al. Science 294(5547):1679-84, 2001

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