ROCK1 antibody RabMAb®

Cat.#: 1953-1
Abcam ID: ab134181

Rabbit Monoclonal Antibody

---

Download datasheet [PDF]|Similar Products|Related Products

Bulk and Custom formulation available Email request

---

Description

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:500 - 1,000
IHC: 1:50 - 100
ICC: 1:50 - 100
IP: 1:60

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

---

Products Data

A. Western blot analysis on HeLa cell lysate using anti-ROCK1 RabMAb (cat. # 1953-1).	B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-ROCK RabMAb (cat. #1953-1).

---

Specificity

A synthetic peptide corresponding to residues near the C-terminus of human ROCK1 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ROCK1 antibody, P160ROCK antibody, ROCK-I antibody, Rho-associated protein kinase 1 antibody, p160 ROCK-1 antibody

Description References

1. Riento K, et al. Nat Rev Mol Cell Biol 4:446-456, 2003.
2. Coleman ML, et al. Nat Cell Biol 3:339-345, 2001.
3. Dvorsky R, et al. J Biol Chem 279:7098-7104, 2004.

Similar Products

Product Name	Catalog #
ROCK1 RabMAb®	1761-1
ROCK1 antibody	S2711

Related Products

Product Name	Catalog #
Goat Anti-Rabbit IgG (H+L) HRP	3053-1
Goat Anti-Rabbit IgG polymerized HRP	3051-1
Cell Lysis Buffer	3500-1
Goat Anti-Rabbit IgG HRP Antibody	3056-1