MIP-1 Beta ELISA Kit antibody 
RabMAb®
Cat.#: 6118-1
Price: $450
A Simple, Complete ELISA Kit
- All-In-One: Quick and easy sandwich ELISA Kit
- Sensitive: Useful for detection of endogenous levels of MIP-1β
Description
The macrophage inflammatory proteins-MIP-1a and MIP-1β are members of the β or CC subfamily of chemokines. Both were initially co-purified from the conditional media of a LPS-stimulated mouse macrophage cell line (1). The mature human MIP-1β and human MIP-1α share 70% homologous in their protein sequences. MIP-1β level in synovial fluids is linked to inflammation diseases. Its levels were increased in synovial fluids from patients with osteoarthritis (18.0 +/- 8.9 ng/ml) compared to patients with rheumatoid arthritis (6.1 +/- 2.9 ng/ml) or other forms of arthritis (10.4 +/- 7.0 ng/ml)(2). Determination of the expression levels of the MIP-1s turned out to provide important information regarding numerous diseases such as multiple myeloma, allergic asthmatic disorders, acute experimental autoimmune encephalomyelitis, HIV infection, sarcoidosis and sepsis. The human MIP-1β ELISA is an enzyme-linked immunosorbant assay for the quantitative detection of MIP-1β levels in cell culture supernatants, serum and plasma. It is also can detect the recombinant MIP-1β. This assay employs the sandwich enzyme immunoassay technique that detects levels of MIP-1β. Rabbit anti- MIP-1β monoclonal antibody has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and MIP-1β bound to the immobilized antibody. Following extensive washing, biotinylated rabbit anti- MIP-1β monoclonal antibody reagent is added. Following washes to remove any unbound antibody reagent, streptavidin-HRP is added to the well. After washing away the unbound streptavidin-HRP, a substrate solution is added to the wells to develop color. The magnitude of the absorbance for this developed color is proportional to the amount of MIP-1β levels. This kit is not intended to be a quantitative ELISA kit.
Recommended Applications
ELISA
Species Reactivity
Products Data
 |  |
| Steps involved in the Mip1-Beta Sandwich ELISA kit. | Example Data. These standard curves are provided for demonstration. A standard curve should be generated for each set of samples assayed |
Components
List of Components :
- Rabbit MIP-1β Monoclonal Anitbody Coated Microwells
- Biotinylated MIP-1β Antibody Reagent (96 reactions, 11mLs)
- Sample & standard Diluent CF (21mLs)
- Sample & Diluent GS (21mLs)
- Lyophilized recombinant human MIP-1β
- Streptavidin-HRP Reagent (100x)
- ELISA Washing Buffer
- TMB A Substrate Solution
- TMB B Substrate Solution
- Stop Solution
Storage Condition and Buffer
NULL
Description References
1. Wolpe, S.D. and A. Cerami (1989) FASEB J. 3:2565 2. Koch, A.E. et al. Clin Immunol Immunopathol. 1995 Dec; 77(3):307-14.
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