Total & Phospho (pT91/93) c-Jun Dual Detection ELISA Kit antibody 
RabMAb®
Cat.#: 6116-1
Price: $450
A Simple, Complete ELISA Kit
- All-In-One: Quick and easy sandwich ELISA Kit
- Sensitive: Useful for detection of endogenous levels of c-Jun and phospho c-Jun pT91/93.
Description
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5-TGA [CG] TCA-3. c-Jun forms homodimers and heterodimers with Fos and other jun-related proteins which, together, comprise the AP-1 transcription factor that binds TPA response elements (TREs). c-Jun therefore mediates transcriptional regulation in response to a variety of stimulants (1). c-Jun is tightly regulated posttranslationally and is phosphorylated in two distinct regions. Ser 63 and 73 are required to be phosphorylated for efficient transactivation function. The kinases responsible for this modification in vivo are the SAPK/JNKs (2, 3). Transactivation of c-Jun is regulated by Jun-N-terminal kinases (JNKs) through phosphorylation at serine 63 and 73 (S63/S73), as well as at threonine 91 and 93 (T91/T93). The integrity of JNK phosphorylation sites at serines 63/73 and at threonines 91/93 in c-Jun is essential for signal-dependent target gene activation (4). These two groups of phosphoacceptor sites respond to different grades of genotoxic stress. c-Jun phosphorylation is restricted to S63/S73, following a short exposure to the DNA-damaging compound etoposide. In contrast, JNK-dependent phosphorylation of T91/T93 requires continuous exposure to the drug (5). This assay employs the sandwich enzyme immunoassay technique that detects endogenous level of c-Jun and phospho C-Jun pT91/93. Rabbit anti-c-Jun monoclonal antibody has been pre-coated onto the microplate. Cell lysate is pipetted into the wells. Following extensive washing, biotinylated rabbit anti-c-Jun antibody and biotinylated rabbit anti-c-Jun Phospho (pT91/93) monoclonal antibody reagent is added. Following a wash to remove any unbound antibody reagent, streptavidin-HRP is added to the well. After washing away the unbound streptavidin-HRP, a substrate solution is added to the wells to develop color. The magnitude of the absorbance for this developed color is proportional to the amount of endogenous total and phosphorylated c-Jun.
Recommended Applications
ELISA
Products Data
 |  |
| Fig 1. Main steps of the Total and Phospho c-Jun (pT91/93) Sandwich ELISA assay. |
Components
- Dry Plate with stripwells (96)
- Biotinylated c-Jun Phospho (pT91/93) Antibody Reagent (48 reactions, 5.5mLs)
- Biotinylated c-Jun Total Antibody Reagent (48 reactions, 5.5mLs)
- Streptavidin-HRP Reagent
- Antigen/Antibody Diluent Buffer
- ELISA Washing Buffer
- TMB A Substrate Solution
- TMB B Substrate Solution
- Stop Solution
Storage Condition and Buffer
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