ZAP-70 Total ELISA Kit antibody 
RabMAb®
Cat.#: 6114-1
Price: $450
A Simple, Complete ELISA Kit
- All-In-One: Quick and easy sandwich ELISA Kit
- Sensitive: Useful for detection of levels of total Zap-70
Description
ZAP-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Lck phosphorylates Tyrosine 492 and 493 located at the activation loop of the catalytic domain of ZAP-70, leading to its activation and increased autophosphorylation (3). This assay employs the sandwich enzyme immunoassay technique that detects endogenous levels of ZAP-70. Rabbit anti-ZAP-70 monoclonal antibody has been pre-coated onto a microplate. Cell lysates are pipetted into the wells. Following extensive washing, biotinylated rabbit anti-ZAP-70 monoclonal antibody reagent is added to detect ZAP-70. Following a wash to remove any unbound antibody reagent, streptavidin-HRP is added to the well. After washing away the unbound streptavidin-HRP, TMB substrate is added to the wells to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of ZAP-70 protein
Recommended Applications
ELISA
Products Data
 |  |
| Fig 1. Main steps of the ZAP-70 Total Sandwich ELISA assay. |
Components
- Dry Plate with stripwells (96)
- Biotinylated ZAP-70 Antibody Reagent
- Streptavidin-HRP Reagent
- Antigen/Antibody Diluent Buffer
- ELISA Washing Buffer
- TMB A Substrate Solution
- TMB B Substrate Solution
- Stop Solution
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.Related Products
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