PBK ELISA Kit antibody 
RabMAb®
Cat.#: 6112-1
Price: $450
A Simple, Complete ELISA Kit
- All-In-One: Quick and easy sandwich ELISA Kit
- Sensitive: Useful for detection of levels of endogenous PBK
Description
PDZ-binding kinase (PBK) or TOPK, an intermediate in the Ras-MAPK Kinase signaling, is upregulated in a variety of neoplasms including hematological malignancies. Studies provide a plausible explanation for the role of PBK augmenting tumor cell growth following transient appearance in different types of progenitor cells in vivo (1). PBK is a newly identified member of a novel MEK3/6-related MAPKK that may be enrolled in the activation of lymphoid cells and support testicular functions (2). In vitro, PBK binds specifi cally to PDZ2 of hDlg through its C-terminal T/SXV motif. PBK and hDlg are phosphorylated at mitosis in HeLa cells, and the mitotic phosphorylation of PBK is required for its kinase activity. In vitro, cdc2/cyclin B phosphorylates PBK. This evidence shows how PBK could link hDlg or other PDZ-containing proteins to signal transduction pathways regulating the cell cycle or cellular proliferation (3). This assay employs the sandwich enzyme immunoassay technique that detects endogenous levels of PBK (Fig 1). Rabbit anti-PBK monoclonal antibody has been pre-coated onto a microplate (Step 1). Cell lysate is pipetted into the wells (Step 2). Following extensive washing, biotinylated rabbit anti-PBK monoclonal antibody reagent is added (Step 3). Following a wash to remove any unbound antibody reagent, streptavidin-HRP is added to the well (Step 4). After washing away the unbound streptavidin-HRP, a substrate solution is added to the wells to develop color (Step 5). The magnitude of the absorbance for this developed color is proportional to the amount of endogenous PBK protein. This kit is not intended to be a quantitative ELISA kit.
Recommended Applications
ELISA
Products Data
 |  |
| Fig. 1. Main steps of the PBK Sandwich ELISA Kit. |
Components
- Dry Plate with stripwells (96)
- Biotinylated PBK Antibody Reagent
- Streptavidin-HRP Reagent
- Antigen/Antibody Diluent Buffer
- ELISA Washing Buffer
- TMB A Substrate Solution
- TMB B Substrate Solution
- Stop Solution
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.Related Products
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