c-Jun ELISA Kit antibody 
RabMAb®
Cat.#: 6111-1
Price: $450
A Simple, Complete ELISA Kit
- All-In-One: Quick and easy sandwich ELISA Kit
- Sensitive: Useful for detection of endogenous levels of c-Jun
Description
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5-TGA[CG]TCA-3. c-Jun forms homodimers and heterodimers with Fos and other jun-related proteins which, together, comprise the AP-1 transcription factor that binds TPA response elements (TREs). c-Jun therefore mediates transcriptional regulation in response to a variety of stimulants (1). c-Jun is tightly regulated posttranslationally and is phosphorylated in two distinct regions. Ser 63 and 73 are required to be phosphorylated for effi cient transactivation function. The kinases responsible for this modification in vivo are the SAPK/JNKs (2,3). This assay employs the sandwich enzyme immunoassay technique that detects endogenous levels of c-Jun (Fig 1). Rabbit anti-c-Jun monoclonal antibody has been pre-coated onto a microplate (Step 1). Cell lysate is pipetted into the wells (Step 2). Following extensive washing, biotinylated rabbit anti-c-Jun monoclonal antibody reagent is added (Step 3). Following a wash to remove any unbound antibody reagent, streptavidin-HRP is added to the well (Step 4). After washing away the unbound streptavidin-HRP, a substrate solution is added to the wells to develop color (Step 5). The magnitude of the absorbance for this developed color is proportional to the amount of endogenous c-Jun protein.
Recommended Applications
ELISA
Products Data
 |  |
| Fig1. Main steps of the c-Jun Sandwich ELISA Kit. |
Components
- Dry Plate with stripwells (96)
- Biotinylated c-Jun Antibody Reagent
- Streptavidin-HRP Reagent
- Antigen/Antibody Diluent Buffer
- ELISA Washing Buffer
- TMB A Substrate Solution
- TMB B Substrate Solution
- Stop Solution
Storage Condition and Buffer
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