Atg3 (ATG3) antibody RabMAb®

Cat.#: 3686-1
Abcam ID: ab108282

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).

Recommended Applications

WB, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IP: 1:10 - 100
FC: 1:10 - 100

Species Reactivity *

Human, Mouse, Rat

*Species cross-reactivity is based on WB analysis

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Products Data

Western blot analysis on (A) K562, (B) HeLa, and (C) Jurkat cell lysates using anti-Atg3 RabMAb (cat. #3686-1).	Flow cytometric analysis of permeabilized Jurkat cells using anti-Atg3 RabMAb (red) (catalog # 3686-1) or a rabbit IgG (negative) (green).

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Specificity

A synthetic peptide corresponding to residues on the C-terminus in human Atg3 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ATG3 antibody, APG3 antibody, APG3-LIKE antibody, APG3L antibody, PC3-96 antibody, Ubiquitin-like-conjugating enzyme ATG3 antibody, Protein PC3-96 antibody

Description References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010).

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