RasGap Phospho (pY460) (RASA1) antibody 
RabMAb®
Cat.#: 3633-1
Rabbit Monoclonal Antibody
Swiss Prot: P20936
Mol Weight: 110-120kDa
Price: $285*
Availability: Ship next business day
Description
RasGap, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGap is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGap is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGap promotes apoptosis (4).
Recommended Applications
WB, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
ICC: 1:100 - 250
FC: 1:10 - 100
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis
Products Data
 |  |
| Western blot analysis on 293T cell lysates using anti-Phospho-RasGap (pY460) RabMAb (cat. #3633-1). Cells were either (A) untreated (B) treated with Pervanadate. | Immunofluorescent staining of 293T cells using anti-Phospho-RasGap (pY460) RabMAb (cat. #3633-1). |
Specificity
A phospho specific peptide corresponding to residues surrounding Tyrosine 460 of human RasGap was used as an immunogen. This antibody only detects RasGap phosphorylated at Tyrosine 460.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RASA1 antibody, CM-AVM antibody, CMAVM antibody, DKFZp434N071 antibody, GAP antibody, PKWS antibody, RASA antibody, RASGAP antibody, p120GAP antibody, p120RASGAP antibody, Ras GTPase-activating protein 1 antibody, Ras p21 protein activator antibody, p120GAP antibody
Description References
1. Yang, J.-Y., Michod, D., Walicki, J., and Widmann, C. (2004) Biochem. Pharmacol. 68, 1027
2. Yang, J.-Y., and Widmann, C. (2001) Mol. Cell. Biol. 21, 5346-5358
3. Yang, J.-Y., Michod, D., Walicki, J., Murphy, B. M., Kasibhatla, S., Martin, S., and Widmann, C. (2004) Mol. Cell. Biol. 24, 10425
4. Yang, J. Y., Walicki, J., Michod, D., Dubuis, G., and Widmann, C. (2005) Mol. Biol. Cell, 16, 3511
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