LIN28A antibody 
RabMAb®
Cat.#: 3533-1
Rabbit Monoclonal Antibody
Swiss Prot: Q9H9Z2
Mol Weight: 26kDa
Price: $260*
Availability: Ship next business day
Description
LIN28A, a promoter of pluripotency, is a developmentally regulated RNA binding protein, which selectively blocks the processing of pri-let-7 miRNAs in embryonic cells (1,2). One of the let-7 family members, let-7g targets and represses LIN28 through four 3'-UTR binding sites. It has been reported that LIN28/let-7 pair has the potential to act as a 'toggle switch' that balances the decision to maintain pluripotency vs. differentiation (3). LIN28 was identified as a negative regulator of miRNA biogenesis and suggested that it may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (1).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100
FC: 1:10 - 100
Species Reactivity *
Human
*Species cross reactivity determined by western blot analysis only.
Products Data
 |  |  |
| Western blot analysis on (A) NCCIT and (B) JAR cell lysates using anti-LIN28A RabMAb (cat. #3533-1). | Immunohistochemical analysis of paraffin-embedded human seminoma tissue using anti-LIN28A (Cat. #3533-1) RabMAb. | Immunofluorescent staining of NCCIT cells using anti-LIN28A (Cat. #3533-1) RabMAb. |
Specificity
A synthetic peptide corresponding to residues in human LIN28A was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
LIN28A antibody, CSDD1 antibody, FLJ12457 antibody, LIN-28 antibody, LIN28 antibody, ZCCHC1 antibody, Protein lin-28 homolog A antibody, Zinc finger CCHC domain-containing protein 1 antibody
Description References
1. Viswanathan SR, et al. Science 320(5872):97-100, 2008
2. Büssing I, et al. Trends Mol Med 14(9):400-9, 2008
3. Gunaratne PH, et al. Curr Stem Cell Res Ther20094(3):168-77
Similar Products
| Product Name | Catalog # |
|---|---|
| LIN28 RabMAb® | 3334-1 |
| LIN28A DyLight® 488 RabMAb® | 4584-1 |
| LIN28A DyLight® 594 RabMAb® | 4585-1 |
Related Products
| Product Name | Catalog # |
|---|---|
| Goat Anti-Rabbit IgG (H+L) HRP | 3053-1 |
| Goat Anti-Rabbit IgG polymerized HRP | 3051-1 |
| Cell Lysis Buffer | 3500-1 |
| Goat Anti-Rabbit IgG HRP Antibody | 3056-1 |
| Goat Anti-Rabbit IgG DyLight® 405 | 3063-1 |
| Goat Anti-Rabbit IgG DyLight® 488 | 3064-1 |
| Goat Anti-Rabbit IgG DyLight® 550 | 3065-1 |
| Goat Anti-Rabbit IgG DyLight® 594 | 3066-1 |
| Goat Anti-Rabbit IgG DyLight® 633 | 3067-1 |
| Goat Anti-Rabbit IgG DyLight® 650 | 3068-1 |
