Beta-Tubulin class III (C-term) (TUBB3) antibody RabMAb®

Cat.#: 1967-1
Abcam ID: ab52623

Rabbit Monoclonal Antibody

---

Download datasheet [PDF]|Similar Products|Related Products

Bulk and Custom formulation available Email request

---

Description

Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3).

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:50 - 100
ICC: 1:100 - 250
IP: 1:40
FC: 1:10

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

---

Products Data

A. Western blot analysis on HeLa cell lysate using anti-Beta-Tubulin class III RabMAb (cat. # 1967-1).	B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Tubulin beta class III RabMAb (cat# 1967-1)

---

Specificity

A synthetic peptide corresponding to the C-term of human class III β-Tubulin was used as immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

TUBB3 antibody, CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, Tubulin beta-3 chain antibody, Tubulin beta-4 chain antibody, Tubulin beta-III antibody

Description References

1. Wang, HW et al. Nature 435: 911-915, 2005.
2. Cleveland, DW et al. Cell 20: 95-105, 1980.
3. Hasegawa S et al. Clin Can Res 9: 2992-2997, 2003.

Similar Products

Product Name	Catalog #
Beta-Tubulin class III (N-term) RabMAb®	2276-1

Related Products

Product Name	Catalog #
Goat Anti-Rabbit IgG (H+L) HRP	3053-1
Goat Anti-Rabbit IgG polymerized HRP	3051-1
Cell Lysis Buffer	3500-1
Goat Anti-Rabbit IgG HRP Antibody	3056-1