ARF1 antibody RabMAb®

Cat.#: 1635-1
Abcam ID: ab32524

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase, and it recruits COPI to membranes (4-5).

Recommended Applications

WB, IHC, FC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:250-500
FC: 1:80

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

A. Western blot analysis on 293 cell lysate using anti-ARF1 RabMAb (cat. # 1635-1), dilution 1:2,000.	B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-ARF1 RabMAb (cat. # 1635-1).

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Specificity

A synthetic peptide corresponding to residues in human ARF1 was used as immunogen. This antibody is predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ARF1 antibody, ADP-ribosylation factor 1 antibody

Description References

1. Bobak et al. Proc. Nat. Acad. Sci. 86: 6101-6105, 1989.
2. Serafini, T., Orci, L., Amherdt, M., Brunner, M., Kahn, R. A., and Rothman, J. E. (1991) Cell 67, 239
3. Yang, C. Z., Heimberg, H., D\'Souza-Schorey, C., Mueckler, M. M., and Stahl, P. D. (1998) J. Biol. Chem. 273, 4006
4. Bonifacino, J.S., and J. Lippincott-Schwartz. 2003. Nat. Rev. Mol. Cell Biol. 4:409
5. Nie, Z., D.S. Hirsch, and P.A. Randazzo. 2003. Arf and its many interactors. Curr. Opin. Cell Biol. 15:396

Product References

1. LuoWB, Wang YF,and Reiser G, p24A, a Type I Transmembrane Protein, Controls ARF1-dependent Resensitization of Protease-activated Receptor-2 by Influence on Receptor Trafficking ,The Journal of Biological Chemistry 282 (2007) 30246–30255
[Application: WB]

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