Adducin alpha (C-term) (ADD1) antibody 
RabMAb®
Cat.#: 1706-1
Rabbit Monoclonal Antibody
Swiss Prot: P35611
Mol Weight: 130kDa
Price: $260*
Availability: Ship next business day
Description
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:5,000-50,000
IHC: 1:250-500
ICC: 1:250-500
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |  |  |
| A. Western blot analysis on Hela cell lysate using anti-K562 RabMAb (cat. # 1706-1), dilution 1:50,000. | B. Immunohistochemical analysis of paraffin-embedded human lung using anti-Adducin (C-term) RabMAb (cat. # 1706-1). | C. Immunofluorescent staining of HeLa using anti-Adducin RabMAb (C-term) (cat. # 1706-1). |
Specificity
A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ADD1 antibody, ADDA antibody, MGC3339 antibody, MGC44427 antibody, Alpha-adducin antibody, Erythrocyte adducin subunit alpha antibody
Description References
1. Gardner, K.; Bennett, V. : J. Biol. Chem. 261: 1339-1348, 1986.
2. Kuhlman, P.A., C.A. Hughes, V. Bennett, and V.M. Fowler. 1996. J. Biol. Chem. 271: 7986-7991
3. Dong, L., C. Chapline, B. Mousseau, L. Fowler, K. Ramsay, J.L. Stevens, and S. Jaken. 1995. J. Biol. Chem. 270: 25534-25540
4. Palfrey, H. C., Waseem, A. (1985) J. Biol. Chem. 260, 16021-16029
5. Fukata et al. J. Cell Biol., Volume 145, Number 2, April 19, 1999 347-361
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