Akt Substrates Ab Sampler Kit
Cat.#: 5005-1Price: $535
A sampler kit with rabbit monoclonal antibodies (RabMAbs) specific to proteins directly modified by Akt (14-3-3, Bad, Chk1, GSK3, IKK-a, p27 (Kip1), Raf). This Kit is provided with sufficient amount of antibodies for multiple immunoassays.
Description
AKT (Protein Kinase B) signaling pathway regulates a variety of biological processes including survival, proliferation, cell growth, cell motility and glycogen metabolism. Specifically, Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2).
Recommended Applications
WB, IHC, ICC, IP, FC
Species Reactivity
Human, Mouse, Rat
Products Data
 |  |
| A-G. Assay data using Akt Substrates Ab Sampler Kit (Cat# 5005-1) on various human samples. |
Specificity
Human, Mouse, Rat
Components
Antibodies to: 14-3-3, Bad, Chk1, GSK3, IKK-a, p27 (Kip1), Raf
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Description References
1. Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599 2. Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318
