Calponin-1 (CNN1) antibody RabMAb®

Cat.#: 1806-1
Abcam ID: ab46794

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

A. Western blot analysis on human bladder lysate using anti-Calponin RabMAb (cat. # 1806-1).	B. Immunohistochemical staining of paraffin-embedded human smooth muscle using anti-Calponin RabMAb (cat. # 1806-1).	C. Immunofluorescent staining of Hela cells using anti Calponin-1 RabMAb(cat. # 1806-1).

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Specificity

A synthetic peptide corresponding to residues near the C-term of human Calponin was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

CNN1 antibody, SMCC antibody, Sm-Calp antibody, Calponin-1 antibody, Basic calponin antibody, Calponin H1, smooth muscle antibody

Description References

1. Strasser P, et al. FEBS Lett. 330(1):13-8, 1993
2. Ferjani I, et al. FEBS Lett. 580(20):4801-6, 2006
3. Prasad AR, et al. Arch Pathol Lab Med. 123(9): 801-6, 1999

Product References

1. Guo XY, Oshima H, Kitmura T, et al, Stromal Fibroblasts Activated by Tumor Cells Promote Angiogenesis in Mouse Gastric Cancer ,J. Biol. Chem. 283 (2008) 19864 - 19871
[Application: IHC]

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