ROCK1 antibody

Cat.#: S2711
Abcam ID: ab97592

Rabbit Polyclonal Antibody

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Description

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]

Recommended Applications

WB, IHC

Applications and Recommended Dilution Factors

WB: 1:500-1:3000
IHC: 1:100-1:500

Species Reactivity *

Human

*Other species have not been tested

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Products Data

Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Molt-4 5% SDS PAGE diluted at 1:1000	Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using ROCK1 antibody at 1:500 dilution.

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Specificity

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 107 and 381 of ROCK1

Storage Condition and Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names

ROCK1 antibody, P160ROCK antibody, ROCK-I antibody, Rho-associated protein kinase 1 antibody, p160 ROCK-1 antibody

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