ATP-citrate lyase Phospho (pS455) (ACLY) antibody RabMAb®

Cat.#: 1822-1
Abcam ID: ab46796

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).

Recommended Applications

WB, IP

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IP: 1:20

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on 3T3 cell lysates using anti-Phospho-ACL (pS455) RabMAb (cat. #1822-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with insulin.

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Specificity

A synthetic peptide corresponding to residues of human ATP citrate lyase was used as immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ACLY antibody, ACL antibody, ATPCL antibody, CLATP antibody, ATP-citrate synthase antibody, Citrate cleavage enzyme antibody

Description References

1. Elshourbagy et al. Europ. J. Biochem. 204: 491-499, 1992.
2. Benjamin, W. B., and Singer, I. (1974) Biochim. Biophys. Acta 251, 28-422.

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