Vimentin DyLight^® 488 (VIM) antibody RabMAb®

Cat.#: 4528-1
Abcam ID: ab139855

Rabbit Monoclonal Antibody

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Description

Vimentin is the most common member of the intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in a variety of mesenchymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).

Recommended Applications

ICC, FC

Applications and Recommended Dilution Factors

ICC: 1:100 - 200
FC: 1:50

Species Reactivity

Human

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Products Data

Immunofluorescent analysis of of HeLa cells using Vimentin RabMAb (DyLight 488 conjugate) (green) and Dapi (blue).	Flow cytometric analysis of Jurkat cells using Vimentin RabMAb (DyLight 488 conjugate) (red) compared to a nonspecific negative control antibody (green).

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Specificity

A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.

Storage Condition and Buffer

Supplied in PBS (pH 7.2), 0.02% sodium azide, 0.05% BSA. Store at 4℃. Protect from light. Stable for 6 months from date of receipt.

Alternative Names

VIM antibody, Vimentin antibody

Description References

1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992. 2. Inagaki M et al. Nature 328: 649-652, 1987. 3. Goto H et al. GENES CELLS 7:91-97. 2002. 4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998. 5. Yamaguchi T et al. JCB, 171(3):431-436, 2005. DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

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