ROCK1 antibody RabMAb®

Cat.#: 1761-1
Abcam ID: ab45171

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:50 - 100
ICC: 1:250 - 500
IP: 1:50

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis anti-ROCK1 RabMAb on untreated HeLa cell lysate (A) and camptothecin treated HeLa lysate (B). (cat. # 1761-1).	B. Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-ROCK1 RabMAb (cat # 1761-1).	C. Immunofluorescent staining of HeLa cells using anti-ROCK1 RabMAb (cat. # 1761-1).

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Specificity

A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen. This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ROCK1 antibody, P160ROCK antibody, ROCK-I antibody, Rho-associated protein kinase 1 antibody, p160 ROCK-1 antibody

Description References

1. Riento K, et al. Nat Rev Mol Cell Biol 4:446-456, 2003.
2. Coleman ML, et al. Nat Cell Biol 3:339-345, 2001.
3. Dvorsky R, et al. J Biol Chem 279:7098-7104, 2004.

Product References

1. Zucchini-Pascal N, de Sousa G, Pizzol J, et al, Pregnane X receptor activation protects rat hepatocytes against deoxycholic acid-induced apoptosis ,Liver Int 30 (2010) 284-297
[Application: WB]

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