Histone H3T Acetyl (K27) (HIST1H3J) antibody RabMAb®Cat.#: 1767-1
Abcam ID: ab45173
Rabbit Monoclonal Antibody
Swiss Prot: P68431
Mol Weight: 17kDa
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5).
WB, IHC, ICC
Applications and Recommended Dilution Factors
IHC: 1:100 - 250
ICC: 1:100 - 250
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
|A. Western blot analysis on C6 cell lysate using anti-Histone H3T Acetyl (K27) RabMAb (cat. #1767-1), 1:500 dilution. Cells were either (1) untreated or (2) treated with TSA.||B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-H3T Acetyl-K27 RabMAb. (cat. # 1767-1).||C. Immunofluorescent staining of A431 cells using anti-Histone H3T Acetyl-K27 RabMAb. (cat. # 1767-1).|
A synthetic acetylated peptide corresponding to residues surrounding Lys 27 of Histone H3T was used as immunogen. The antibody cross-reacts with Histone H2B acetylated on Lysine 5.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
HIST1H3J antibody, H3/j antibody, H3FJ antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody
1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Albig W, et al. Hum Genet 97:486-491. 1996.
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