Histone H3 Acetyl (K18) (HIST1H3J) antibody RabMAb®

Cat.#: 1766-1
Abcam ID: ab40888

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

A. Western blot analysis on C6 cell lysate using anti-Histone H3 Acetyl (K18) RabMAb (cat. #1766-1), 1:50,000 dilution. Cells were (1) untreated or (2) treated with TSA.	B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti--Histone H3 Acetyl (K18) RabMAb (cat. # 1766-1).

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Specificity

A synthetic acetylated peptide corresponding to residues surrounding Lys18 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 18.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

HIST1H3J antibody, H3/j antibody, H3FJ antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

Description References

1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Ahn SH, et al. Cell Cycle 4:780-783, 2005.

Product References

1. Vlaicu SI, Tegla CA, Cudrici CD, et al, Epigenetic modifications induced by RGC-32 in colon cancer ,Exp Mol Pathol 88 (2010) 67-76
[Application: WB]

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