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14-3-3 gamma antibody

Cat.#: S2085
Abcam ID: ab137804

Rabbit Polyclonal Antibody

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Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]

Recommended Applications

WB, IHC

Applications and Recommended Dilution Factors

WB: 1:500-1:3000
IHC: 1:100-1:500

Species Reactivity *

Human

*Other species have not been tested

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Products Data

Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000	Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using 14-3-3 gamma antibody at 1:500 dilution.

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Specificity

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 241 of 14-3-3 gamma

Storage Condition and Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names

YWHAG antibody, 14-3-3GAMMA antibody, 14-3-3 protein gamma, N-terminally processed antibody, Protein kinase C inhibitor protein 1 antibody

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