Src Phospho (pY529) (SRC) antibody 
RabMAb®
Cat.#: 1550-1
Rabbit Monoclonal Antibody
Swiss Prot: P12931
Mol Weight: 60kDa
Price: $285*
Availability: Ship next business day
Description
Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3) Mutation of Y529 leads to Src overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).
Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:5,000-10,000
ICC: 1:50
IP: 1:50
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A. Western blot analysis on A431 cell lysate using anti-Phospho-Src (pY529) RabMAb (cat. # 1550-1), dilution 1:10,000. Cells were either (A) untreated, (B) treated with EGF and (C) treated with EGF+ alkaline phosphatase. | B. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-Src (pY529) RabMAb (cat. # 1550-1) dilution 1:2,000 | C. Immunofluorescent staining of A431 cells using anti-Phospho-Src (pY529) RabMAb (cat. # 1550-1). |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 529 of human Src was used as immunogen. The antibody will detect Src phosphorylation on Tyrosine 529 of both isoforms.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
SRC antibody, ASV antibody, SRC1 antibody, c-SRC antibody, p60-Src antibody, Proto-oncogene tyrosine-protein kinase Src antibody, Proto-oncogene c-Src antibody, pp60c-src antibody
Description References
1. Cooper, J. A., and King, C. S. (1986) Mol. Cell. Biol. 6, 4467-4477
2. Courtneidge, S. A. (1985) EMBO J. 4, 1471-1477
3. Okada, M., Howell, B. W., Broome, M. A., and Cooper, J. A. (1993) J. Biol. Chem. 268, 18070-18075
4. Leiming Li, Masaya Okura, Akira Imamoto MOLECULAR AND CELLULAR BIOLOGY, Feb. 2002, p. 1203
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