RSK1 Phospho (pT359/pS363) (RPS6KA1) antibody RabMAb®

Cat.#: 2006-1
Abcam ID: ab32413

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:100
ICC: 1:50
IP: 1:40

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

Immunohistochemical staining of untreated paraffin-embedded human cervical carcinoma using anti-Phospho-Rsk1 (pT359/pS363) RabMAb (cat. #2006-1).	Immunohistochemical staining of Phosphatase-treated paraffin-embedded human cervical carcinoma using anti-Phospho-Rsk1 (pT359/pS363) RabMAb (cat. #2006-1).	Western blot analysis on A431 cell lysate using anti- Phospho-Rsk1 (pT359/pS363) RabMAb (cat. #2006-1). Cell lysates were (1) untreated, or (2) treated with EGF.

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Specificity

A synthetic phospho-peptide corresponding to residues surrounding Thr359 and Ser363 of human RSK1 was used as immunogen. The antibody only detects RSK1 phosphorylated on Threonine 359 and Serine 363.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

RPS6KA1 antibody, HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Ribosomal protein S6 kinase alpha-1 antibody, Ribosomal S6 kinase 1 antibody

Description References

1. Dalby, K.N. et al. Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK. J. Biol. Chem. 273, 1496-1505 (1998)
2. Frodin, M. and Gammeltoft, S. Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction. Mol. Cell. Endocrinol. 151, 65
3. Lazar, D. F. et al. Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin. J. Biol. Chem. 270, 20801
4. Chen, R. et al. Phosphorylation of the c-Fos Transrepression Domain by Mitogen-Activated Protein Kinase and 90-kDa Ribosomal S6 Kinase. Proc. Natl. Acad. Sci. USA 90, 10952

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