RasGap (RASA1) antibody 
RabMAb®
Cat.#: 1671-1
Rabbit Monoclonal Antibody
Swiss Prot: P20936
Mol Weight: 110-120kDa
Price: $260*
Availability: Ship next business day
Description
RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:2,000
IHC: 1:50
ICC: 1:250-500
Species Reactivity *
Human, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A. Western blot analysis on Rat brain lysate using anti-RasGAP RabMAb (cat. # 1671-1), dilution 1:2,000. | B. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-RasGap RabMAb (cat. # 1671-1). | C. Immunofluorescent staining of HeLa cells using anti-RasGAP RabMAb (cat. # 1671-1). |
Specificity
A synthetic peptide corresponding to residues near the SH3 domain of human RasGAP was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RASA1 antibody, CM-AVM antibody, CMAVM antibody, DKFZp434N071 antibody, GAP antibody, PKWS antibody, RASA antibody, RASGAP antibody, p120GAP antibody, p120RASGAP antibody, Ras GTPase-activating protein 1 antibody, Ras p21 protein activator antibody, p120GAP antibody
Description References
1. Yang, J.-Y., Michod, D., Walicki, J., and Widmann, C. (2004) Biochem. Pharmacol. 68, 1027
2. Yang, J.-Y., and Widmann, C. (2001) Mol. Cell. Biol. 21, 5346-5358
3. Yang, J.-Y., Michod, D., Walicki, J., Murphy, B. M., Kasibhatla, S., Martin, S., and Widmann, C. (2004) Mol. Cell. Biol. 24, 10425
4. Yang, J. Y., Walicki, J., Michod, D., Dubuis, G., and Widmann, C. (2005) Mol. Biol. Cell, 16, 3511
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