LRRK2 antibody RabMAb®

Cat.#: 3516-1
Abcam ID: ab133476

Rabbit Monoclonal Antibody

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Description

Well-characterized antibodies to efficiently detect and purify LRRK2 protein are a critical need in the Parkinson's Disease (PD) research community. To help accelerate LRRK2 research, The Michael J. Fox Foundation (MJFF), working with Epitomics, Inc., has generated unique and high quality LRRK2 rabbit monoclonal antibodies to be widely available for PD research community.

LRRK2 (Leucine-rich repeat kinase 2, dardarin) is a protein kinase belonging to the ROCO family, which is defined by the presence of a ROC (Ras/GTPase of complex proteins) domain and COR (C-terminal of Roc) region (1). LRRK2 exhibits kinase activity whereby it can undergo autophosphorylation and can phosphorylate generic substrates. In addition, the GTPase domain of LRRK2 can mediate GDP (guanosine-5′-diphosphate)/GTP (guanosine-5′-triphosphate) binding as well as GTP hydrolysis. (2).

LRRK2 is mutated in a significant number of Parkinson's disease (PD) patients. Mutations in this gene account for 4% of PD, and are observed in 1% of sporadic PD patients (4). Clinical symptoms of patients carrying PD-associated mutations of LRRK2 are indistinguishable from typical sporadic PD. The spectra of neuropathological features of PARK8 (type 8), the type corresponding to LRRK2, is broad and appears to encompass those associated with other familial PD cases such as PARK1 (alpha-synuclein) and PARK2 (Parkin) (5). Patients with this gene mutation have typical relatively late onset Parkinsonism with features comparable with idiopathic PD; symptoms include asymmetric rest tremor, bradykinesia, rigidity, and a good response to 3,4-dihyroxy-l-phenylalanine (l-DOPA). The pathology of cases with LRRK2 mutations is pleomorphic (6).

For more characterization data and protocols using this LRRK2 Antibody, please visit PD Online Research Site.

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:1000 - 10,000
IHC: 1:200 - 500
ICC: 1:50
IP: 2 - 5 ug

Species Reactivity *

Human, Mouse, Rat

*Species cross-reactivity is based on WB analysis

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Products Data

A. Western blot analysis on Jurkat cell lysate using anti-LRRK2 RabMAb (cat. #3516-1). Lane1: HEK293 cell lysate transfected with 3*Flag vector. Lane2: HEK293 cell lysate transfected with 3*Flag-wild type hLRRK2_full length. Data provided by Zhuohua Zhang Lab (Sanford-Burnham Medical Research Institute).

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Specificity

A synthetic peptide corresponding to aa 970 - aa 2527 of human LRRK2 was used as an immunogen.

Storage Condition and Buffer

Tissue culture supernatant: PBS with 0.01% Azide. Store at 4℃.

Alternative Names

LRRK2 antibody, AURA17 antibody, DARDARIN antibody, PARK8 antibody, RIPK7 antibody, ROCO2 antibody, Leucine-rich repeat serine/threonine-protein kinase 2 antibody, Dardarin antibody

Description References

1. J Neurosci. 2010 Feb 3;30(5):1788-97.
2. PLoS Genet. 2010 Apr 8;6(4):e1000902.
3. BMB Rep. 2010 Apr;43(4):233-44.
4. Biochem J. 2010 Aug 27;430(3):393-404.
5. FEBS J. 2009 Nov;276(22):6445-54. Epub 2009 Oct 5.
6. Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18676-81. Epub 2005 Dec 13.

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