14-3-3 b/a (C-term) (YWHAB) antibody RabMAb®

Cat.#: 1062-1
Abcam ID: ab32560

Rabbit Monoclonal Antibody

---

Download datasheet [PDF]|Similar Products|Related Products

Bulk and Custom formulation available Email request

---

Description

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000
IHC: 1:500
ICC: 1:500
IP: 1:40
FC: 1:40

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

---

Products Data

A. Western blot analysis on Hela cell lysate using anti-14-3-3 b/a (C-term) RabMAb (cat. #1062-1), dilution 1:1,000.	B. Immunohistochemical analysis of paraffin-embedded breast tissue anti-14-3-3 b/a (C-term) RabMAb (cat. #1062-1).

---

Specificity

A synthetic peptide corresponding to C-terminal residues of human 14-3-3 b/a was used as immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

YWHAB antibody, GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, 14-3-3 protein beta/alpha, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody

Description References

1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619

Similar Products

Product Name	Catalog #
14-3-3 b/a (N-term) RabMAb®	1058-1
14-3-3 beta antibody	S2600

Related Products

Product Name	Catalog #
Goat Anti-Rabbit IgG (H+L) HRP	3053-1
Goat Anti-Rabbit IgG polymerized HRP	3051-1
Cell Lysis Buffer	3500-1
Goat Anti-Rabbit IgG HRP Antibody	3056-1