Thiophosphate Ester (WB version) antibody RabMAb®Cat.#: 2686-1
Abcam ID: ab92570
Rabbit Monoclonal Antibody
Swiss Prot: N/A
This novel rabbit monoclonal antibody (RabMAb®) can be used to detect kinase substrates containing thiophosphate esters following the kinase reaction as described by J. Allen and colleagues in Nature methods 2007 (Figure 1). This antibody allows researchers to follow this approach to study kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs (1).
This product is recommended for WB usage.
Applications and Recommended Dilution Factors
WB: 1:1000 - 10,000
This antibody is specific to thiophosphorylated amino acids.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
1. Allen, J.J. et al. Nature Methods, 4(6), 511-516, 2007.
1. Carlson SM, Chouinard CR, et al, Large-Scale Discovery of ERK2 Substrates Identifies ERK-Mediated Transcriptional Regulation by ETV3 ,Sci Signal. (2011) 4(196):rs11
2. Carlson HK, Plate L, Price MS, et al, Use of a semisynthetic epitope to probe histidine kinase activity and regulation ,Anal Biochem 397 (2010) 139-143
3. huluunbaatar U, Roller R, Feldman ME, et al, Constitutive mTORC1 activation by a herpesvirus Akt surrogate stimulates mRNA translation and viral replication ,Genes Dev 24 (2010) 2627-2639
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