IKK beta (IKBKB) antibody RabMAb®

Cat.#: 1601-1
Abcam ID: ab32135

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

IKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquitination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta (3). Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response (4). Additionally, IKK-beta activity can also be regulated by MEKK-1 (5).

Recommended Applications

WB, IHC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 5,000
IHC: 1:50-100
IP: 1:50
FC: 1:40

Species Reactivity *

Human, Mouse

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on Daudi cell lysate using anti-IKK-beta RabMAb (cat. #1601-1) 1:5,000 dilution.	B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-IKK RabMAb (cat. # 1601-1).

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Specificity

A synthetic peptide corresponding to residues near the C-term of human IKK-beta was used as immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

IKBKB antibody, IKK-beta antibody, IKK2 antibody, IKKB antibody, NFKBIKB antibody, Inhibitor of nuclear factor kappa-B kinase subunit beta antibody, Nuclear factor NF-kappa-B inhibitor kinase beta antibody

Description References

1. Zandi, E.; Rothwarf, D. M.; Delhase, M.; Hayakawa, M.; Karin, M.: Cell 91: 243-252, 1997
2. Scheidereit, C. Docking IB kinase. Nature 395: 225-226, 1998
3. Woronicz, J. D.; Gao, X.; Cao, Z.; Rothe, M.; Goeddel, D. V.: Science 278: 866-869, 1997.
4. Delhase, M.; Hayakawa, M.; Chen, Y.; Karin, M.: Science 284: 309-312, 1999.
5. Nakano, H., M. Shindo, S. Sakon, S. Nishinaka, M. Mihara, H. Yagita, and K. Okumura. Proc. Natl. Acad. Sci. USA 95: 3537-3542, 1998

Product References

1. Reyna SM, Ghosh S, Tantiwong P, et al, Elevated Toll-Like Receptor 4 Expression and Signaling in Muscle From Insulin-Resistant Subjects ,Diabetes 57 (2008) 2595 - 2602
[Application: IP]
2. Ghosh S, Choudary A, Ghosh S, et al, IKKβ Mediates Cell Shape-Induced Aromatase Expression and Estrogen Biosynthesis in Adipose Stromal Cells ,Mol Endocrinol 23 (2009) 662-670
[Application: WB]

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