p53 Phospho (pS33) (TP53) antibody 
RabMAb®
Cat.#: 2262-1
Rabbit Monoclonal Antibody
Swiss Prot: P04637
Mol Weight: 53kDa
Price: $285
Availability: Ship next business day
Description
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5). Cyclin dependent kinase 9, whose well-known substrate is RNA polymerase II, can also phosphorylate p53, more specifically, Ser33 on the N-terminus and, Ser315 and Ser392 on the C-terminus (6).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:1000
ICC: 1:100
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on T47D cell lysates using anti-Phospho-p53 (pS33) RabMAb (cat. #2262-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with etoposide. | B. Immunofluorescent staining of A431 cells using anti-Phospho-p53 RabMAb (cat. # 2262-1). |
Specificity
A phospho specific peptide corresponding to residues surrounding serine 33 of human p53 was used as an immunogen. This antibody detects p53 phosphorylated at serine 53.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
1. Levine, A.J. et al. Cell 88: 323
2. Lakin, N.D. et al. Oncogene 18: 7644
3. Vogelstein, B. et al. Nature 348: 681
4. Hoolstein, M., et al. Science 253: 49
5. Harris C.C. Science 262: 1980
6. Radhakrishnan SK, et al. Cell Cycle 5(5):519-21, 2006
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