YAP65 Phospho (pS127) (YAP1) antibody 
RabMAb®
Cat.#: 2209-1
Rabbit Monoclonal Antibody
Swiss Prot: P46937
Mol Weight: 65 - 75kDa
Price: $285
Availability: Ship next business day
Description
The transcriptional coactivator Yes-associated protein (YAP, YAP65) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). YAP binds with 14-3-3 after being phosphorylated by Akt at Ser127. Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2).
Recommended Applications
WB, IHC
Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1:100 - 250
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on HeLa cell lysates using anti-Phospho-YAP65 (pS127) RabMAb (cat. #2209-1), 1:50,000 dilution. Cells were either (A) untreated (B) treated with FBS + calyculin A. | B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-YAP65 RabMAb (cat# 2209-1). |
Specificity
A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP65 was used as immunogen. The antibody detects YAP65 phosphorylated at Serine 127.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
YAP1 antibody, YAP antibody, YAP2 antibody, YAP65 antibody, YKI antibody, Yorkie homolog antibody, 65 kDa Yes-associated protein antibody
Description References
1. Strano S, et al. Mol Cell 18(4):447-59, 2005
2. Basu S, et al. Mol Cell 11(1):11-23, 20033.
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