ZAP70 (C-term) DyLight® 488 antibody 
RabMAb®
Cat.#: 4524-1
Rabbit Monoclonal Antibody
Swiss Prot: P43403
Mol Weight: 70kDa
Price: $275*
Availability: Ship next business day
Description
Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).
Recommended Applications
FC
Applications and Recommended Dilution Factors
FC: 1:50
Species Reactivity *
Human
*DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Products Data
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| Flow cytometric analysis of Jurkat cells using ZAP-70 RabMAb (DyLight 488 conjugate) (red) compared to a nonspecific control antibody (green). |
Specificity
A synthetic peptide corresponding to residues in C-terminus of human ZAP-70 was used as immunogen.
Storage Condition and Buffer
Supplied in PBS (pH 7.2), 0.02% sodium azide, 0.05% BSA. Store at 4℃. Protect from light. Stable for 6 months from date of receipt.
Alternative Names
ZAP70 antibody, FLJ17670 antibody, FLJ17679 antibody, SRK antibody, STD antibody, TZK antibody, ZAP-70 antibody, Tyrosine-protein kinase ZAP-70 antibody, 70 kDa zeta-associated protein antibody, Syk-related tyrosine kinase antibody
Description References
1. Chu, D.H., et al. Immunol. Rev. 165: 167-180 (1998). 2. Isakov, N., et al. J Exp Med. 181: 375-80 (1995). 3. Di Bartolo, V., et al. J Biol Chem. 274: 6285-94 (1999).
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