p53 Acetyl (K305) (TP53) antibody 
RabMAb®
Cat.#: 3308-1
Rabbit Monoclonal Antibody
Swiss Prot: P04637
Mol Weight: 53kDa
Price: $285*
Availability: Ship next business day
Description
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers. It is acetylated at lysine 305, which is a p300 acetyltransferase-mediated acetylation site (4).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis
Products Data
 |
| Western blot analysis on HepG2 cell lysates using anti-p53 Acetyl (K305) RabMAb (cat. #3308-1). Cells were either (A) untreated (B) treated with etoposide and TSA. |
Specificity
A synthetic acetylated peptide corresponding to residues surrounding Lysine 305 of human p53 was used as an immunogen. The antibody only detects p53 acetylated on Lysine 305.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
1. Levine, A.J. Cell. 1997 Feb 7;88(3):323-31.
2. Lakin, N.D et al. Oncogene. 1999 Dec 13;18(53):7644-55.
3. Vogelstein, B. Nature. 1992 19 March;356, 215 - 221.
Wang YH et al. J Biol Chem. 2003 Jul 11;278(28):25568-76.
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