ZAP70 Phospho (pY292) DyLight® 488 antibody 
RabMAb®
Cat.#: 4515-1
Rabbit Monoclonal Antibody
Swiss Prot: P43403
Mol Weight: 70kDa
Price: $275*
Availability: Ship next business day
Description
ZAP70, a Syk-family protein tyrosine kinase (PTK), plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP70 via its tandem SH2 domains. This binding correlates with activation of ZAP70, a critical event in T cell activation (2). Following TCR engagement, ZAP70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck (3). Heterologous trans- phosphorylation of Tyrosine 493 by Src-PTKs is required for antigen receptor-mediated activation of both the calcium and Ras pathways. In contrast however, cells expressing mutations at Tyrosine 292 or 492 demonstrate hyperactive T- and B-cell antigen receptor phenotypes. Thus, phosphorylation of ZAP-70 mediates both activation and inactivation of antigen receptor signaling (4).
Recommended Applications
FC
Applications and Recommended Dilution Factors
FC: 1:50
Species Reactivity *
Human
*DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Products Data
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| Flow cytometric analysis of Jurkat cells, untreated (red) and treated with pervanadate (blue) using Zap70 Phospho (pY292) RabMAb (DyLight 488 conjugate) compared to a negative control antibody (green). |
Specificity
A phospho-specific peptide corresponding to residues surrounding Tyrosine 292 of human ZAP70 was used as an immunogen. This antibody only detects ZAP70 phosphorylated on Tyrosine 292
Storage Condition and Buffer
Supplied in PBS (pH 7.2), 0.02% sodium azide, 0.05% BSA. Store at 4℃. Protect from light. Stable for 6 months from date of receipt.
Alternative Names
ZAP70 antibody, FLJ17670 antibody, FLJ17679 antibody, SRK antibody, STD antibody, TZK antibody, ZAP-70 antibody, Tyrosine-protein kinase ZAP-70 antibody, 70 kDa zeta-associated protein antibody, Syk-related tyrosine kinase antibody
Description References
1. Chu, D.H., et al. Immunol. Rev. 165: 167 2. Isakov, N., et al. J Exp Med. 181: 375 3. Di Bartolo, V., et al. J Biol Chem. 274: 6285 4. G Kong, et al. Mol. Cell. Biol. 16(9):5026-5035, 1996 28378 4. Tremblay L., et al. Int. J. Cancer, 68: 164-171, 1996
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