Sca-1 / Ly-6A/E (Ly6a) antibody RabMAb®Cat.#: 3121-1
Abcam ID: ab109211
Rabbit Monoclonal Antibody
Swiss Prot: P05533
Mol Weight: 18kDa
Sca-1 / Ly-6A/E (stem cell antigen-1) is a glycosylphosphatidylinositol-anchored membrane protein known for its role in T-cell activation, and it can be used as a marker for regeneration-competent myoblasts (1). It is a parenchymal stem cell marker that is used extensively to enrich murine hematopoietic stem cells (2). Moreover, studies have implicated Sca-1 / Ly-6A/E and related family members in cell-cell adhesion among thymocytes (1). Sca-1 / Ly-6A/E has also been reported as a marker for muscle-derived stem cells, and Sca-1 / Ly-6A/E-positive myogenic precursor cells appear to be recruited from the circulation to sites of myocardial injury (1).
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50 - 100
|Western blot analysis on mouse kidney cell lysate using anti-Sca-1 / Ly-6A/E RabMAb (cat. # 3121-1).||Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti- Sca-1 / Ly-6A/E RabMAb (cat. #3121-1).|
A synthetic peptide corresponding to residues in mouse Sca-1 / Ly-6A/E was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Ly6a antibody, Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, Lymphocyte antigen 6A-2/6E-1 antibody, Stem cell antigen 1 antibody
1. Epting CL et al. J Cell Sci. 117(Pt 25):6185-95, 2004. 2. Holmes C and Stanford WL. Stem Cells. 25(6):1339-47, 2007.
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