Polyethylene Glycol (PEG) Backbone antibody RabMAb®

Cat.#: 3104-1
Abcam ID: ab133471

Rabbit Monoclonal Antibody

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Download datasheet [PDF]

Bulk and Custom formulation available Email request

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Description

Polyethylene glycol（PEG）is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs (2,3). The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions (4). As examples, PEG–adenosine deaminase (Adagen®) is used for the treatment of severe combined immunodeficiency syndrome, PEG–asparaginase (Oncaspar®) is used for the treatment acute lymphoblastic leukemia, PEG–interferon α2a (Pegasys®) is used for the treatment Hepatitis C, Branched PEG–anti–VEGF aptamer (Pegaptanib, Macugen™) is used for the treatment Macular degeneration(age-related) (5). An Anti–PEG antibody can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion. In addition, it can be used for the quality control of pegylated molecules in ELISA, WB and flow cytometery.

Please note, this antibody is Rabbit Monoclonal IgM.

Recommended Applications

WB, ELISA

Applications and Recommended Dilution Factors

WB: 1:1000
ELISA:

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Products Data

Various concentrations of PEG polymers with amine end groups were coated in 96-wells. 2 ug/ml of anti-PEG antibody was added. After washing, 1 ug/ml of anti-rabbit IgM-HRP was incubated	Various concentrations of PEG20K conjugated MBP and branched PEG conjugated BSA were coated in 96-wells. 2 ug/ml of anti-PEG antibody was added. After washing, 1 ug/ml of anti-rabbit IgM-HRP was incubated	10 ug/ml of anti-PEG antibody (cat# 3104-1) was coated into 96-wells. Serial dilution of PEG20K conjugated MBP and PEGylated ADI were added. After washing, 1 ug/ml of biotinylated anti-PEG antibody (cat. # 2137-1) was added. Streptavidin-HRP was then used to develop the color.
1 ug (lane 1) and 0.5 ug (lane 2) of MBP-PEG20K and 1 ug (lane 3) and 0.5 ug (lane 4) of PEG20K were electrophoresed on a 4-20% gel, transferred to nitrocellulose membrane, and probed with anti-PEG antibody

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Specificity

KLH-PEG20K was used as an immunogen. This antibody recognizes the backbone of the PEG molecule. Please note, this antibody is Rabbit Monoclonal IgM.

Storage Condition and Buffer

Store at 4°C or aliquot and store at -20 °C. Buffer: PBS, 0.01% sodium azide. Stable for 12 months from date of receipt.

Description References

1. Guiotto, A. et al. (2004) Anchimeric assistance effect on regioselective hydrolysis of branched PEGs: a mechanistic investigation. Bioorg. Med.Chem. 12, 5031–5037 2. Wong, S.S. (1991) Reactive groups of proteins and their modifying agents. In Chemistry of protein conjugation and cross-linking, p. 13, CRC Press 3. Caliceti, P. et al.(1993) Active site protection of proteolytic enzymes by poly(ethylene glycol) surface modification. J. Bioact. Comp. Polym. 8,41–50 4. Frank Leenders, celares GmbH, Berlin, Germany.(2006) PEGylation technology and biopharmaceuticals. Biopharmaceuticals. 6,39-40 5. Francesco M.Veronese, Gianfranco Pasut. (2005) PEGylation, successful approach