Oct-4 (POU5F1) antibody 
RabMAb®
Cat.#: 2876-1
Rabbit Monoclonal Antibody
Swiss Prot: Q01860
Mol Weight: 45kDa
Price: $260*
Availability: Ship next business day
Description
Octamer transcription factor-4 (Oct-4) is a homeodomain transcription factor in the POU transcription factor family that specifically binds to the octamer motif (5'-ATTTCAT-3') (1). It is critical for early embryogenesis and for embryonic stem cell pluripotency, expressed mainly in the developing brain (1). OCT4 is a master regulator that affects the fate of pluripotent stem cells and germ cell precursors (2). For instance, the downregulation of Oct-4 triggers embryonic stem cell differentiation, simultaneous with gastrulation (3). It is also used as a marker for germ cell tumor diagnosis (4).
Recommended Applications
WB, IHC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:500 - 1,000
IP: 1:10 - 100
FC: 1:10 - 100
Species Reactivity
Human
Products Data
 |  |
| Western blot analysis on (A) Seminoma, (B) NCCIT, and (C) HeLa cell lysates using anti-Oct-4 RabMAb (cat. # 2876-1). | Immunohistochemical staining of paraffin-embedded human ovarian dysgerminoma using anti-Oct-4 RabMAb (cat. #2876-1). |
Specificity
A synthetic peptide corresponding to residues in human Oct-4 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
POU5F1 antibody, MGC22487 antibody, OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU domain, class 5, transcription factor 1 antibody, Octamer-binding transcription factor 3 antibody
Description References
1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2010). 2. Wei F et al. J Biol Chem. 282(29):21551-60, 2007. 3. Pesce et al. Stem Cells. 19(4):271-8, 2001. 4. Cheng et al. J Pathol. 211(1):1-9, 2007.
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