IKK alpha (N-term) (CHUK) antibody 
RabMAb®
Cat.#: 1615-1
Rabbit Monoclonal Antibody
Swiss Prot: O15111
Mol Weight: 85kDa
Price: $260
Availability: Ship next business day
Description
IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:50
ICC: 1:500
IP: 1:50
FC: 1:40
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on Daudi cell lysate using anti-IKK-alpha (N-term) RabMAb (cat. #1615-1) 1:50,000 dilution | B. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-IKK alpha (N-term) RabMAb (cat. # 1615-1). |
Specificity
A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CHUK antibody, IKBKA antibody, IKK-alpha antibody, IKK1 antibody, IKKA antibody, NFKBIKA antibody, TCF16 antibody, Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody, Transcription factor 16 antibody
Description References
1. Zandi, E. et al. Cell 91: 243-252, 1997. 2. Scheidereit, C. Nature 395: 225-226, 1998. 3. Ozes, O. N. et al. Nature 401: 82-85, 1999. 4. Anest, V. et al. Nature 423: 659-663, 2003. 5. Yamamoto, Y. et al. Nature 423: 655-659, 2003.
Product References
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