c-Myc Phospho (pT58/pS62) (MYC) antibody 
RabMAb®
Cat.#: 1203-1
Rabbit Monoclonal Antibody
Swiss Prot: P01106
Mol Weight: 57-70kDa
Price: $285*
Availability: Ship next business day
Description
Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).
Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:50-100
IP: 1:100
Species Reactivity *
Human, Mouse
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (catalog #1203-1), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA. | B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAb (catalog #1203-1). |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
MYC antibody, MRTL antibody, bHLHe39 antibody, c-Myc antibody, Myc proto-oncogene protein antibody, Proto-oncogene c-Myc antibody, Transcription factor p64 antibody
Description References
1. Nisen, P.D. et al. Enhanced expression of the N-myc gene in Wilms
2. Nau, M.N. et al. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 318: 69
3. Gupta, S. et al. Transactivation of Gene Expression by Myc is Inhibited by Mutation at the Phosphorylation Sites Thr-58 and Ser-62. Proc. Natl. Acad. Sci. USA 90: 3216
Product References
Similar Products
| Product Name | Catalog # |
|---|---|
| c-Myc (N-term) RabMAb® | 1472-1 |
| c-Myc antibody | S1242 |
| Phospho-MYC-T58 Antibody | T3831 |
| c-Myc DyLight® 488 RabMAb® | 4581-1 |
Related Products
| Product Name | Catalog # |
|---|---|
| Goat Anti-Rabbit IgG (H+L) HRP | 3053-1 |
| Goat Anti-Rabbit IgG polymerized HRP | 3051-1 |
| Cell Lysis Buffer | 3500-1 |
| Goat Anti-Rabbit IgG HRP Antibody | 3056-1 |
| Goat Anti-Rabbit IgG DyLight® 405 | 3063-1 |
| Goat Anti-Rabbit IgG DyLight® 488 | 3064-1 |
| Goat Anti-Rabbit IgG DyLight® 550 | 3065-1 |
| Goat Anti-Rabbit IgG DyLight® 594 | 3066-1 |
| Goat Anti-Rabbit IgG DyLight® 633 | 3067-1 |
| Goat Anti-Rabbit IgG DyLight® 650 | 3068-1 |
