Alpha-Tubulin (TUBA1B) antibody RabMAb®

Cat.#: 2871-1
Abcam ID: ab108629

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Microtubules are essential to eukaryotic cytoskeletal structures, vesicular transportation, and mitosis. It consists mainly of two soluble protein subunits, alpha- and beta-tubulin. Alpha-tubulin binds to beta-tubulin to form a heterodimer that is subject to post-translation modifications (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta-tubulin, but not alpha-tubulin. The stability of the dimer in the microtubules is dependent on the presence of beta-tubulin, since the dimer with GTP bound beta-tubulin is stable in microtubule integration (2). For both types of tubulins, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at the microtubule surface (3). Defects in alpha-tubulin are the cause of lissencephaly type 3 (LIS3), characterized by agyria (4).

Recommended Applications

WB, IHC, ICC, FC

Applications and Recommended Dilution Factors

WB: 1:10,000-50,000
IHC: 1: 500 - 1,000
ICC: 1: 250 - 500
FC: 1: 10 - 100

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

Western blot analysis on (A) HeLa, (B) Jurkat, (C) 293T, and (D) A431 cell lysates using anti-Alpha-Tubulin RabMAb (cat. # 2871-1).	Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Alpha-Tubulin RabMAb (cat. #2871-1).	Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Alpha-Tubulin RabMAb (cat. #2871-1).
Immunofluorescent staining of HeLa cells using anti-Alpha-Tubulin RabMAb (cat. #2871-1).

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Specificity

A synthetic peptide corresponding to residues in human Alpha-Tubulin was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

TUBA1B antibody, K-ALPHA-1 antibody, Tubulin alpha-1B chain antibody, Alpha-tubulin ubiquitous antibody, Tubulin K-alpha-1 antibody, Tubulin alpha-ubiquitous chain antibody

Description References

1. Wang HW et al. Nature 435(7044): 911-915, 2005.
2. Cleveland DW et al. Cell 20: 95-105, 1980.
3. Westermann S et al. Nat. Rev. Mol. Cell Biol. 4:938 -947, 2003.
4. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009).

Product References

1. Nagamine T, Shimomura S, Sueyoshi N, et al, Influence of Ser/Pro-rich domain and kinase domain of double cortin-like protein kinase on microtubule-binding activity ,J Biochem 149 (2011) 619-627

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