ZAP-70 (ZAP70) antibody 
RabMAb®
Cat.#: 2869-1
Rabbit Monoclonal Antibody
Swiss Prot: P43403
Mol Weight: 70kDa
Price: $260*
Availability: Ship next business day
Description
ZAP-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell antigen receptor (TCR) activation (1). Its involvement in pre-TCR and TCR signaling affects the adaptive immune response and thymocyte development (2). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with the recruitment of ZAP-70, a critical event in T cell activation (3). Following TCR engagement, ZAP-70 is phosphorylated by several tyrosine residues in the activation loop of the catalytic domain (2). Phosphorylation of Tyr315 and Tyr319 is required for full activation and increased signal propagation of downstream target molecules by ZAP-70 (4).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1:250 - 500
ICC: 1: 250 - 500
IP: 1: 10 - 100
Species Reactivity
Human
Products Data
 |  |
| Western blot analysis on (A) human T-78 and (B) Jurkat cell lysates using anti-ZAP-70 RabMAb (cat. # 2869-1). | Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP-70 RabMAb (cat. #2869-1). |
Specificity
A synthetic peptide corresponding to residues in human ZAP-70 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ZAP70 antibody, FLJ17670 antibody, FLJ17679 antibody, SRK antibody, STD antibody, TZK antibody, ZAP-70 antibody, Tyrosine-protein kinase ZAP-70 antibody, 70 kDa zeta-associated protein antibody, Syk-related tyrosine kinase antibody
Description References
1. Chu DH et al. Immunol Rev. 165:167-80, 1998. 2. Deindl S et al. Proc Natl Acad Sci U S A., 10.1073/pnas.0911512106, 2009. 3. Isakov N et al. J Exp Med. 181(1):375-80, 1995. 4. Di Bartolo V et al. J Biol Chem. 274(10):6285-94, 1999.
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