p27/Kip1 (C-term) (CDKN1B) antibody 
RabMAb®
Cat.#: 1591-1
Rabbit Monoclonal Antibody
Swiss Prot: P46527
Mol Weight: 27kDa
Price: $260
Availability: Ship next business day
Description
p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100-250
ICC: 1:100-250
IP: 1:50
FC: 1:40
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A. Western blot analysis on MCF-7 cell lysate using anti-p27 (Kip1) RabMAb (cat. #1591-1), 1:1,000 dilution. | B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-p27 (Kip1) (C-term) RabMAb (cat. # 1591-1). | C. Immunofluorescent staining of PC-12 cells using anti-P27 (Kip1) (C-term) RabMAb (cat. # 1591-1). |
Specificity
A synthetic peptide corresponding to residues in the C-term of human p27(Kip1) was used as immunogen
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CDKN1B antibody, CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, Cyclin-dependent kinase inhibitor 1B antibody, Cyclin-dependent kinase inhibitor p27 antibody, p27Kip1 antibody
Description References
1. Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., Tempst P., Massague J.; Cell 78:59-66 (1994).
2. Carrano,A.C., Eytan,E., Hershko,A. and Pagano,M. (1999) SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol., 1, 193-199
3. Rodier,G., Montagnoli,A., Di Marcotullio,L., Coulombe,P., Draetta,G.F., Pagano,M. and Meloche,S. (2001). EMBO J., 20, 6672-6682
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