Chk1 (CHEK1) antibody 
RabMAb®
Cat.#: 2865-1
Rabbit Monoclonal Antibody
Swiss Prot: O14757
Mol Weight: 56kDa
Price: $220*
Availability: Ship next business day
Description
Chk1 is a serine/threonine-protein kinase required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA (1). It is regulated by Atr and plays an important role in embryonic development and tumor suppression (2). The activated Chk1 triggers the G2/M checkpoint, which arrests the cells in G2 to allow them to repair their DNA (3). The inhibition of Chk1 kinase results in the abrogation of the G2/M checkpoint and leads to cell death (3). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (4). Activated Chk1 can bind to, phosphorylate (at serine 216), and inactivate cdc25c (4, 5).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
Species Reactivity
Human
Products Data
 |
| Western blot analysis on (A) Jurkat (B) K562, (C) MCF-7, and (D) HepG52 cell lysates using anti-Chk1 RabMAb (cat. # 2865-1). |
Specificity
A synthetic peptide corresponding to residues in human Chk1 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CHEK1 antibody, CHK1 antibody, Serine/threonine-protein kinase Chk1 antibody, antibody
Description References
1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009). 2. Liu et al. Genes Dev. 14(12): 1448-1459, 2000. 3. Tao ZF et al. Anticancer Agents Med Chem. 6(4):377-88, 2006. 4. Zhao et al. Mol Cell Biol 21:4129-4139, 2001. 5. Zeng et al. Nature 395:507-510, 1998.
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