GSK3 (C-term) (GSK3B) antibody 
RabMAb®
Cat.#: 1561-1
Rabbit Monoclonal Antibody
Swiss Prot: P49841
Mol Weight: 46kDa
Price: $260
Availability: Ship next business day
Description
Glycogen synthase kinase 3beta (GSK-3β) is a Serine/Threonine protein kinase involved in insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3β activity is downregulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Alzheimer's disease. Additionally, increased GSK-3β protein levels are found in Alzheimer's disease brains.
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:100-250
ICC: 1:100-250
Species Reactivity *
Human, Mouse
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on A431 cell lysate using anti- GSK-3 beta (C-term) RabMAb (cat. # 1561-1), dilution 1:10,000. | B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-GSK-3 beta (C-term) RabMAb (cat. # 1561-1). |
Specificity
A synthetic peptide corresponding to residues in the C-term of human GSK-3β was used as immunogen. This antibody may detect splice isoform 2 based on sequence homology.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
GSK3B antibody, - antibody, Glycogen synthase kinase-3 beta antibody, antibody
Description References
1. Plyte SE, Hughes K, Nikolakaki E, Pulverer BJ, Woodgett JR. Biochim Biophys Acta. 1992 Dec 16; 1114(2-3):147
2. Miller, J. R. & Moon, R. T. (1996) Genes Dev. 10, 2527-2539
3. Boyle WJ, Smeal T, Defize LH, Angel P, Woodgett JR, Karin M, Hunter T. Cell. 1991 Feb 8; 64(3):573
4. Bellacosa, A., Testa, J. R., Staal, S. P. & Tsichllis, P. N. (1992) Science 254, 244-247
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