Raf-1 (RAF1) antibody 
RabMAb®
Cat.#: 1560-1
Rabbit Monoclonal Antibody
Swiss Prot: P04049
Mol Weight: 74kDa
Price: $260
Availability: Ship next business day
Description
Raf-1 (c-Raf) is a protein kinase that is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Raf-1 is recruited by and binds to GTP-bound Ras, activating the MEK-MAP kinase pathway (1). Raf-1 is activated via rapid hyperphosphorylation at multiple sites in mammalian cells, including Ser43, Ser259, and Ser621 (2), as well as Ser338, Tyr341, Thr491, and Ser494 (3). p21-activated protein kinase (PAK) phosphorylates Raf-1 at Ser338 in response to Ras, Src and epidermal growth factor (4).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50-100
ICC: 1:50
IP: 1:100
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on NIH 3T3 cell lysate using anti-Raf-1 RabMAb (cat. #1560-1). | B. Immunohistochemical analysis of paraffin- embedded human colon carcinoma using anti-Raf-1 RabMAb RabMab (catalog #1560-1). |
Specificity
A synthetic peptide corresponding to residues surrounding Serine 338-339 of human Raf was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RAF1 antibody, CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, Raf-1 antibody
Description References
1. Avruch, J., et al. Raf meets Ras: completing the framework of a signal transduction pathway. Trends Biochem. Sci. 19: 279
2. Morrison, D.K., et al. Identification of the major phosphorylation sites of the Raf-1 kinase. J Biol Chem. 268: 17309
3. Chong, H., et al. Positive and negative regulation of Raf kinase activity and function by phosphorylation. EMBO J. 20: 3716
4. King, A.J., et al. The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338. Nature 396: 180
Product References
1. Yu CF, Han WL, Shi TP, et al, PTPIP51, a novel 14-3-3 binding protein, regulates cell morphology and motility via Raf-ERK pathway ,Cellular Signalling 20 (2008) 2208-2220
[Application: IP]
2. Fan S, Meng Q, Laterra JJ, et al, Ras effector pathways modulate scatter factor-stimulated NF-κB signaling and protection against DNA damage ,Oncogene 26 (2007) 4774–4796
[Application: WB]
3. Alejandro EU, Kalynyak TB, Taghizadeh F, et al, Acute Insulin Signaling in Pancreatic Beta-Cells Is Mediated by Multiple Raf-1 Dependent Pathways ,Endocrinology 151 (2010) 502-512
4. Hsu SP, Lee WS, Progesterone Receptor Activation of Extranuclear Signaling Pathways in Regulating p53 Expression in Vascular Endothelial Cells ,Mol Endocrinol 25 (2011) 421-432
Similar Products
| Product Name | Catalog # |
|---|---|
| RAF1 antibody | S1535 |
| RAF1 antibody | S2099 |
| Phospho-RAF1-pS338 Antibody | T3855 |
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