Bad (N-term) (BAD) antibody 
RabMAb®
Cat.#: 1541-1
Rabbit Monoclonal Antibody
Swiss Prot: Q92934
Mol Weight: 23kDa
Price: $260
Availability: Ship next business day
Description
Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:250 - 500
IP: 1:100
FC: 1:50
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |  |  |
| A.Western blot analysis on Hela cell lysate using anti-Bad (N-term) RabMAb (cat. # 1541-1), dilution 1:5,000. | B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-Bad (N-term) RabMAb (cat. # 1541-1) | C. Immunohistochemical staining of paraffin-embedded human lymphoma using a anti-Bad (N-term) RabMAb (cat. # 1541-1) |
Specificity
A synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen. The antibody does not cross-react with other Bcl-2 members.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
BAD antibody, BBC2 antibody, BCL2L8 antibody, Bcl2 antagonist of cell death antibody, Bcl-XL/Bcl-2-associated death promoter antibody
Description References
1. Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413
2. Feng J, Funk WD, Wang SS, et al. Science 1995; 269:1236-41
3. Greider, C.W. (1996). Telomere length regulation. Annu. Rev. Biochem. 65, 337-365
4. Shay JW, Cacchetti SA, Eur J Cancer. 1997:33:787-791
Product References
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