Cdc25C Phospho (pS216) (CDC25C) antibody 
RabMAb®
Cat.#: 1190-1
Rabbit Monoclonal Antibody
Swiss Prot: P30307
Mol Weight: 60kDa
Price: $285*
Availability: Ship next business day
Description
Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5).
Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000
ICC: 1:50
IP: 1:30
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on HeLa cell lysates using anti-Phospho-Cdc25C (pS216) RabMAb (cat. #1190-1); dilution 1:1,000. Cells were (1) untreated and (2) treated with Phosphatase. |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C was used as immunogen. The antibody only detects Cdc25C phosphorylated on Serine 216.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CDC25C antibody, CDC25 antibody, M-phase inducer phosphatase 3 antibody, Dual specificity phosphatase Cdc25C antibody
Description References
1. Sadhu, K., et al. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2. Proc Natl Acad Sci U S A. 87: 5139
2. Jessus, C. and R. Ozon. Function and regulation of cdc25 protein phosphate through mitosis and meiosis. Prog. Cell Cycle Res. 1: 215
3. Peng, C.Y., et al. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277: 1501
4. Blasina, A., et al. A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase. Curr Biol. 9: 1
5. Matsuoka, S., et al. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282: 1893
Product References
1. Ye Z, Chen Z, Chen W, et al, XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line ,Chem Biol Interact 183 (2010) 133-141
[Application: WB]
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