Chk1 Phospho (pS280) (CHEK1) antibody 
RabMAb®
Cat.#: 2643-1
Rabbit Monoclonal Antibody
Swiss Prot: O14757
Mol Weight: 56kDa
Price: $285*
Availability: Ship next business day
Description
Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes. Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1). It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4). Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity. Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5).
Recommended Applications
WB, IP
Applications and Recommended Dilution Factors
WB: 1:2500 - 5000
IP: 1:50
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on 293T cell lysates using anti-Phospho-Chk1 (pS280) RabMAb (cat. #2643-1). Cells were either (A) untreated (B) treated with Calyculin A |
Specificity
A phospho-specific peptide corresponding to residues surrounding Serine 280 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated on Serine 280.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CHEK1 antibody, CHK1 antibody, Serine/threonine-protein kinase Chk1 antibody, antibody
Description References
1. Liu Q., et al. Genes Dev. 14:1448-1459, 2000
2. Zhao, H. et al. Mol. Cell. Biol. 21: 4129-4139, 2001
3. Zeng, Y., et al. Nature 395: 507-510, 1998
4. Sanchez, Y., et al. Science 277: 1497-501, 1997
5. The UniProt Consortium, The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195, 2008
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